APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml for 30 minutes at RT |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
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Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human ITGAE protein fragment (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD103 , HUMINAE , ITGAE , integrin subunit alpha E , Integrin alpha E , Integrin αE
Cellular Localization
Membrane
Background
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain, yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin, this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL), and in addition to a role in adhesion, it may serve as an accessory molecule for IEL activation. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX18035 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD103 Mouse Monoclonal Antibody (ITGAE/2063). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX18035 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX18035 Integrin alpha E antibody [ITGAE/2063].
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GTX18035 IHC-P Image
IHC-P analysis of human colon tissue using GTX18035 Integrin alpha E antibody [ITGAE/2063].
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GTX18035 IHC-P Image
IHC-P analysis of human small intestine tissue using GTX18035 Integrin alpha E antibody [ITGAE/2063].
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REFERENCE
There are currently no references for Integrin alpha E antibody [ITGAE/2063] (GTX18035). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Integrin alpha E antibody [ITGAE/2063] (GTX18035). Be the first to share your experience with this product.